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elabscience cat e el m0676  (Elabscience Biotechnology)


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    Elabscience Biotechnology elabscience cat e el m0676
    Elabscience Cat E El M0676, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+hmgb+1+elisa+kit/Mouse%3FHMGB-1+(High%3FMobility%3FGroup%3FProtein%3FB1)%3FELISA%3FKit/pmc13049686-153-30-30
    Average 95 stars, based on 124 article reviews
    elabscience cat e el m0676 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: HMGB1 inhibition blocks ferroptosis and oxidative stress to ameliorate sepsis‐induced acute lung injury by activating the Nrf2 pathway
    Article Snippet: The levels of interleukin‐1β (IL‐1β) and IL‐18 in serum and MLE‐12 cell supernatants were quantified using ELISA kits specific for each cytokine (R&D, USA). .. Similarly, the HMGB1 levels in both serum and MLE‐12 cell supernatants were assayed using a mouse HMGB‐1 ELISA Kit (Elabscience, Wuhan, China). .. The absorbance at 450 nm was recorded using a microplate reader (Bio‐Rad, Hercules, CA).

    Article Title: Mature dendritic cell-derived dendrosomes swallow oxaliplatin-loaded nanoparticles to boost immunogenic chemotherapy and tumor antigen-specific immunotherapy
    Article Snippet: For the quantitative evaluation of ICD markers, CT26 cells were seeded in 6-well plates (2 × 10 5 cells/well) and incubated with 1.0 mL of complete medium for 24 h. The cells were then treated with NP/mDs and each component for another 12 h. Afterward, the cells were collected, incubated with Alexa Fluor 488-CRT antibody and Alexa Fluor 488-Hsp70 antibody (Novus Biologicals, USA), respectively, and then counterstained with PI, and analyzed using flow cytometry. .. CT26 cells were seeded in 6-well plates (2 × 10 5 cells/well) and incubated with 1.0 mL of complete medium for 24 h. The cells were then treated with NP/mDs and each component for another 24 h. Afterward, the cell supernatant was collected and extracellular ATP and HMGB1 concentrations were determined by ATP Determination Kit (A22066, Thermo Fisher Scientific, USA) and Mouse HMGB-1 ELISA Kit (E-EL-M0676c, Elabscience Biotechnology, China), respectively, according to the manufacturer protocols with a Hybrid Multi-Mode Reader. ..

    Article Title: USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis
    Article Snippet: Human IL-18 ELISA Kit (ab215539), Mouse IL-18 ELISA Kit (ab216165), Human IL-1β ELISA Kit (ab214025), Mouse IL-1β ELISA Kit (ab197742), Mouse S100A8 ELISA Kit (ab263886), Mouse S100A9 ELISA Kit (ab213887) and HSP70 ELISA Kit (ab133060) were purchased from Abcam, UK. .. Human S100A8 (E-EL-H1289c), Human S100A9 (E-EL-H1290c), HSP90 ELISA Kit (E-EL-H1864c), Human HMGB-1 ELISA Kit (E-EL-H1554c), Mouse HMGB-1 ELISA Kit (E-EL-M0676c) were obtained from Elabscience Biotechnology Co., Ltd, China. .. ATP Assay Kit (S0027) was purchased from Beyotime, China.

    Article Title: Tumorous IRE1α facilitates CD8 + T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma.
    Article Snippet: .. ELISA analysis on culture medium of melanoma cells or the blood serum of mice after indicated treatment was performed using the Human IL-6 ELISA Kit (Neobioscience, EHC007.96), Human TNF-α ELISA Kit (Neobioscience, EHC103a.96), Human MIG/CXCL9 ELISA Kit (Neobioscience, EHC114.96), Human IP-10/CXCL10 ELISA Kit (Neobioscience, EHC157.96), Human I-TAC/ CXCL11 ELISA Kit (Neobioscience, EHC084.96), Mouse TNF-α ELISA Kit (Elabscience, E-MSEL-M0002), Mouse IL-2 ELISA Kit (Elabscience, E-MSEL-M0036), Mouse IL-6 ELISA Kit (Elabscience, E-EL-M0044c), Mouse ELISA Kit MIG/CXCL9 ELISA Kit (Elabscience, E-ELM3077), Mouse IP-10/CXCL10 ELISA Kit (Elabscience, E-EL-M0021c), Mouse I-TAC/CXCL11 ELISA Kit (Elabscience, E-EL-M0056c) and Mouse HMGB-1 ELISA Kit (Elabscience, E-EL-M0676c) according to the manufacturer’s instructions. .. The absorbance (A450 nm) was measured with a plate reader (Bio-Rad).

    Article Title: Systemic administration of polymersomal oncolytic peptide LTX-315 combining with CpG adjuvant and anti-PD-1 antibody boosts immunotherapy of melanoma.
    Article Snippet: Oncolytic peptide LTX-315 while showing clinical promise in treating solid tumors is limited to intratumoral administration, which is not applicable for inaccessible or metastatic tumors.. The cationic and amphipathic nature of oncolytic peptides engenders formidable challenges to developing systems for their systemic delivery.. Here, we describe cRGD-functionalized chimaeric polymersomes (cRGD-CPs) as a robust systemic delivery vehicle for LTX-315, which in combination with CpG adjuvant and anti-PD-1 boost immunotherapy of malignant B16F10 melanoma in mice. cRGD-CPs containing 14.9 wt% LTX-315 (cRGD-CPs-L) exhibited a size of 53 nm, excellent serum stability, and strong and selective killing of B16F10 cells (versus L929 fibroblasts) in vitro, which provoked similar immunogenic effects to free LTX-315 as revealed by release of danger-associated molecular pattern molecules.

    Article Title: Precise Photodynamic Therapy by Midkine Nanobody-Engineered Nanoparticles Remodels the Microenvironment of Pancreatic Ductal Adenocarcinoma and Potentiates the Immunotherapy.
    Article Snippet: Subsequent procedures were conducted following the manufacturer's 127 instructions (Mouse HMGB-1 ELISA Kit, Elabscience Biotechnology Co., Ltd, China). .. Subsequent procedures were conducted following the manufacturer's 127 instructions (Mouse HMGB-1 ELISA Kit, Elabscience Biotechnology Co., Ltd, China). ..

    Article Title: Tumorous IRE1α facilitates CD8 + T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma
    Article Snippet: .. ELISA analysis on culture medium of melanoma cells or the blood serum of mice after indicated treatment was performed using the Human IL-6 ELISA Kit (Neobioscience, EHC007.96), Human TNF-α ELISA Kit (Neobioscience, EHC103a.96), Human MIG/CXCL9 ELISA Kit (Neobioscience, EHC114.96), Human IP-10/CXCL10 ELISA Kit (Neobioscience, EHC157.96), Human I-TAC/CXCL11 ELISA Kit (Neobioscience, EHC084.96), Mouse TNF-α ELISA Kit (Elabscience, E-MSEL-M0002), Mouse IL-2 ELISA Kit (Elabscience, E-MSEL-M0036), Mouse IL-6 ELISA Kit (Elabscience, E-EL-M0044c), Mouse ELISA Kit MIG/CXCL9 ELISA Kit (Elabscience, E-EL-M3077), Mouse IP-10/CXCL10 ELISA Kit (Elabscience, E-EL-M0021c), Mouse I-TAC/CXCL11 ELISA Kit (Elabscience, E-EL-M0056c) and Mouse HMGB-1 ELISA Kit (Elabscience, E-EL-M0676c) according to the manufacturer’s instructions. .. The absorbance (A450 nm) was measured with a plate reader (Bio-Rad).

    Incubation:

    Article Title: Mature dendritic cell-derived dendrosomes swallow oxaliplatin-loaded nanoparticles to boost immunogenic chemotherapy and tumor antigen-specific immunotherapy
    Article Snippet: For the quantitative evaluation of ICD markers, CT26 cells were seeded in 6-well plates (2 × 10 5 cells/well) and incubated with 1.0 mL of complete medium for 24 h. The cells were then treated with NP/mDs and each component for another 12 h. Afterward, the cells were collected, incubated with Alexa Fluor 488-CRT antibody and Alexa Fluor 488-Hsp70 antibody (Novus Biologicals, USA), respectively, and then counterstained with PI, and analyzed using flow cytometry. .. CT26 cells were seeded in 6-well plates (2 × 10 5 cells/well) and incubated with 1.0 mL of complete medium for 24 h. The cells were then treated with NP/mDs and each component for another 24 h. Afterward, the cell supernatant was collected and extracellular ATP and HMGB1 concentrations were determined by ATP Determination Kit (A22066, Thermo Fisher Scientific, USA) and Mouse HMGB-1 ELISA Kit (E-EL-M0676c, Elabscience Biotechnology, China), respectively, according to the manufacturer protocols with a Hybrid Multi-Mode Reader. ..



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    Elabscience Biotechnology hmgb1
    Identification of cell death type mediated by ATP6V1B2 lactylation and GSDME pathway analysis. (A) GSEA enrichment analysis plot of ATP6V1B2 high-expression samples. (B) Annexin V/7-AAD double staining flow cytometry detecting cell death rate. (C) Cell viability (CCK-8) analysis after treatment with ferroptosis inhibitor (Fer-1) and necroptosis inhibitor (NSA). (D) Cellular morphology observation under bright-field microscope. Scale bar: 100 μm. (E) LDH release rate detection. (F, G) Immunoblot analysis of GSDME (F) and GSDMD/GSDMC (G) cleavage. (H–J) ELISA detection of IL-1β (H), IL-18 (I), and <t>HMGB1</t> (J) in cell supernatants. LPS + ATP as positive control. (K) Verification of GSDME and GSDMD siRNA knockdown efficiency. (L) Cellular morphology observation after knockdown of GSDME or GSDMD. (M, N) Analysis of LDH release (M) and cell death rate (N) after knockdown of GSDME. Data are expressed as Mean ± SEM. (n = 3 independent experiments) ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.
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    Elabscience Biotechnology elisa
    Identification of cell death type mediated by ATP6V1B2 lactylation and GSDME pathway analysis. (A) GSEA enrichment analysis plot of ATP6V1B2 high-expression samples. (B) Annexin V/7-AAD double staining flow cytometry detecting cell death rate. (C) Cell viability (CCK-8) analysis after treatment with ferroptosis inhibitor (Fer-1) and necroptosis inhibitor (NSA). (D) Cellular morphology observation under bright-field microscope. Scale bar: 100 μm. (E) LDH release rate detection. (F, G) Immunoblot analysis of GSDME (F) and GSDMD/GSDMC (G) cleavage. (H–J) ELISA detection of IL-1β (H), IL-18 (I), and <t>HMGB1</t> (J) in cell supernatants. LPS + ATP as positive control. (K) Verification of GSDME and GSDMD siRNA knockdown efficiency. (L) Cellular morphology observation after knockdown of GSDME or GSDMD. (M, N) Analysis of LDH release (M) and cell death rate (N) after knockdown of GSDME. Data are expressed as Mean ± SEM. (n = 3 independent experiments) ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.
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    Image Search Results


    Identification of cell death type mediated by ATP6V1B2 lactylation and GSDME pathway analysis. (A) GSEA enrichment analysis plot of ATP6V1B2 high-expression samples. (B) Annexin V/7-AAD double staining flow cytometry detecting cell death rate. (C) Cell viability (CCK-8) analysis after treatment with ferroptosis inhibitor (Fer-1) and necroptosis inhibitor (NSA). (D) Cellular morphology observation under bright-field microscope. Scale bar: 100 μm. (E) LDH release rate detection. (F, G) Immunoblot analysis of GSDME (F) and GSDMD/GSDMC (G) cleavage. (H–J) ELISA detection of IL-1β (H), IL-18 (I), and HMGB1 (J) in cell supernatants. LPS + ATP as positive control. (K) Verification of GSDME and GSDMD siRNA knockdown efficiency. (L) Cellular morphology observation after knockdown of GSDME or GSDMD. (M, N) Analysis of LDH release (M) and cell death rate (N) after knockdown of GSDME. Data are expressed as Mean ± SEM. (n = 3 independent experiments) ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.

    Journal: Redox Biology

    Article Title: Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis

    doi: 10.1016/j.redox.2026.104059

    Figure Lengend Snippet: Identification of cell death type mediated by ATP6V1B2 lactylation and GSDME pathway analysis. (A) GSEA enrichment analysis plot of ATP6V1B2 high-expression samples. (B) Annexin V/7-AAD double staining flow cytometry detecting cell death rate. (C) Cell viability (CCK-8) analysis after treatment with ferroptosis inhibitor (Fer-1) and necroptosis inhibitor (NSA). (D) Cellular morphology observation under bright-field microscope. Scale bar: 100 μm. (E) LDH release rate detection. (F, G) Immunoblot analysis of GSDME (F) and GSDMD/GSDMC (G) cleavage. (H–J) ELISA detection of IL-1β (H), IL-18 (I), and HMGB1 (J) in cell supernatants. LPS + ATP as positive control. (K) Verification of GSDME and GSDMD siRNA knockdown efficiency. (L) Cellular morphology observation after knockdown of GSDME or GSDMD. (M, N) Analysis of LDH release (M) and cell death rate (N) after knockdown of GSDME. Data are expressed as Mean ± SEM. (n = 3 independent experiments) ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001; ns, not significant.

    Article Snippet: Mouse serum total IgE and HDM-specific IgE (R&D Systems), as well as IL-4 (E-EL-M0043), IL-5 (E-EL-M0722), IL-13 (E-EL-M0727), HMGB1 (E-EL-M0676/E-EL-H1554), IL-1β (E-EL-H0149), and IL-18 (E-EL-H0253) in BALF or cell supernatants were all detected using ELISA kits from Elabscience.

    Techniques: Expressing, Double Staining, Flow Cytometry, CCK-8 Assay, Microscopy, Western Blot, Enzyme-linked Immunosorbent Assay, Positive Control, Knockdown